Species Identification of Acanthamoeba Strains Isolated from Patients Referring to Farabi Eye Reference Center Using PCR-RFLP Method
Authors
Abstract:
Introduction: Acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. Infection with Acanthamoeba spp. poses threat to human health, such as, Acanthamoeba keratitis (AK) that is a vision-threatening infection of the cornea. This study aimed to identify the species of Acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. Methods: Acanthamoeba isolates investigated in this cross-sectional study, were collected from patients referring to Farabi Eye Reference Center. All 10 isolates were subjected to species identification using DNA based method. BspLI (NlaIV) and HpyCH4IV restriction enzymes were used to categorize the PCR amplified DNA by PCR-RFLP method. Results: Six samples were identified as Acanthamoeba palestinensis and 4 isolates as Acanthamoeba culbertsoni, which implies that all the isolates belong to pathogenic strains of Acanthamoeba. Conclusion: Acanthamoeba can enter the corneal tissue and survive in the eye, which results in AK. To the authors' knowledge, no study is available on species identification of this genus using these enzymes and technique. This is the first time in Iran that Acanthamoeba isolates are subjected to species identification using PCR-RFLP method.
similar resources
species identification of acanthamoeba strains isolated from patients referring to farabi eye reference center using pcr-rflp method
introduction: acanthamoeba is an opportunistic protist, which is ubiquitously distributed in the environment. infection with acanthamoeba spp. poses threat to human health, such as, acanthamoeba keratitis (ak) that is a vision-threatening infection of the cornea. this study aimed to identify the species of acanthamoeba strains isolated from cornea of keratitis patients by polymerase chain react...
full textIdentification of Malassezia Species Isolated from Patients with Seborrheic Dermatitis Using PCR-RFLP Method in Arak, Iran
Abstract Background and Objective: Malassezia that is a part of normal flora is lipophilic yeast involved in a variety of skin diseases such as seborrheic dermatitis, pityriasis versicolor, atopic dermatitis and psoriasis. Seborrheic dermatitis affects most often the sebaceous-gland-rich areas of skin such as face, scalp, and parts of the upper trunk. Dandruff is a mild variant of seborrheic de...
full textIdentification of Candida species isolated from vulvovaginal candidiasis using PCR-RFLP
Vulvovaginal candidiasis (VVC) is a common disease among women worldwide, therefore, accurate and rapid diagnosis of causative agents based on molecular techniques utilizing amplification of target DNA is highly recomendad for epidemiological purposes and for effective treatment. The aim of this study was to identify clinically Candida species from VVC patients by restriction fragment length po...
full textUse of PCR-RFLP and PCR-HWP1 for identification of Candida species isolated from cystic fibrosis patients
Background: Due to the predisposing conditions in patients with cystic fibrosis (CF) caused by defective mucociliary clearance facilitating colonization and invasion with Candida species has dramatically increased. Traditional methods for identifying problems are imminent and time-consuming. Therefore, molecular techniques utilizing amplification of target DNA provide quick and precise methods ...
full textIdentification of Malassezia Species Isolated from Patients with Pityriasis Versicolor Using PCR-RFLP Method in Markazi Province, Central Iran
BACKGROUND The lipophilic yeasts of Malassezia species are members of the normal skin microbial that are cause of pityriasis versicolor. Pityriasis versicolor is a common superficial fungal infection with world-wide distribution. The phenotypic methods for identification of Malassezia species usually are time consuming and unreliable to differentiate newly identified species. But DNA-based tech...
full textGenotyping of Acanthamoeba Species Isolated from Keratitis Patients by PCR Sequencing Methods in Tehran, Iran
Background and Aims: Amoebae of the genus Acanthamoeba are unicellular amphizoic opportunistic pathogens that may cause fatal granulomatous encephalitis, eye keratitis, amebic pneumonitis and skin nodules as well as abscesses in humans and animals. Acanthamoeba keratitis is caused by trauma to the eye, contaminated cleaning solutions and the use of contact lenses. The aim of the present study w...
full textMy Resources
Journal title
volume 2 issue 3
pages 125- 129
publication date 2014-07
By following a journal you will be notified via email when a new issue of this journal is published.
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023